Počet záznamů: 1

Toward the crystallization of photosystem II core complex from Pisum sativum L

  1. 1.
    0355837 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Prudnikova, T. - Gavira, J. A. - Řezáčová, Pavlína - Molina, E.P. - Hunalová, Ivana - Sviridova, E. - Schmidt, V. - Kohoutová, J. - Kutý, Michal - Kaftan, D. - Vácha, F. - Garcia-Ruiz, J. M. - Kutá-Smatanová, Ivana
    Toward the crystallization of photosystem II core complex from Pisum sativum L.
    Crystal Growth & Design. Roč. 10, č. 8 (2010), s. 3391-3396 ISSN 1528-7483
    Grant CEP: GA MŠk(CZ) LC06010
    Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z50510513; CEZ:AV0Z60870520; CEZ:AV0Z40550506
    Klíčová slova: crystal-structure * cyanobacterial * elongatus
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.390, rok: 2010

    The crystallization of the higher plant similar to 1 gigadalton homodimeric lipid-pigment-protein complex of photosystem II constitutes a challenging undertaking that has not been crowned with success to date. In the continuation of our previous efforts (Kuta-Smatanova et al. Photosyn. Res. 2006, 90, 255-259) that had provided needle and plate crystals of plant photosystem II for the first time, we report on a study to crystallize three-dimensional crystals of the photosystem 11 from Pisum sativum L. suitable for X-ray analysis. In our screening, we optimized temperature, supersaturation rate, and concentrations of detergents, buffers, salts, and organic additives. We used a sitting and hanging drop vapor-diffusion method, microbatch under oil and counter-diffusion techniques, and direct and cross-influenced procedures. We have identified that the major limiting factors for the growth of crystals are the photosystem II stability and requirement of indispensable divalent cations.
    Trvalý link: http://hdl.handle.net/11104/0006317