Počet záznamů: 1

A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin

  1. 1.
    0355821 - UMG-J 2011 RIV DE eng J - Článek v odborném periodiku
    Sobol, Margaryta - Nebesářová, Jana - Hozák, Pavel
    A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin.
    Histochemistry and Cell Biology. Roč. 135, č. 1 (2011), s. 103-110 ISSN 0948-6143
    Grant CEP: GA AV ČR KAN200520704; GA MŠk 2B06063; GA MŠk LC545
    Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z60220518
    Klíčová slova: Mitotic cells * High-pressure freezing * Histochemistry
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.588, rok: 2011

    The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still missing for mitotic cells especially because of their fragility and sensitivity to treatments. Here, we present a fast and effective method for HPF/automated FS and LR White embedding of mitotic cells which allows for their controlled and reproducible quality processing. It should be useful in various ultrastructural studies on mitotic cells especially in combination with immunocytochemistry.
    Trvalý link: http://hdl.handle.net/11104/0194501