Počet záznamů: 1
A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin
0355821 - UMG-J 2011 RIV DE eng J - Článek v odborném periodiku
Sobol, Margaryta - Nebesářová, Jana - Hozák, Pavel
A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin.
Histochemistry and Cell Biology. Roč. 135, č. 1 (2011), s. 103-110 ISSN 0948-6143
Grant CEP: GA AV ČR KAN200520704; GA MŠk 2B06063; GA MŠk LC545
Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z60220518
Klíčová slova: Mitotic cells * High-pressure freezing * Histochemistry
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.588, rok: 2011
The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still missing for mitotic cells especially because of their fragility and sensitivity to treatments. Here, we present a fast and effective method for HPF/automated FS and LR White embedding of mitotic cells which allows for their controlled and reproducible quality processing. It should be useful in various ultrastructural studies on mitotic cells especially in combination with immunocytochemistry.
Trvalý link: http://hdl.handle.net/11104/0194501