Počet záznamů: 1

Equilibrative nucleoside transporter 2 regulates olfactory memory and synaptic function in Drosophila

  1. 1.
    0355637 - BC-A 2011 RIV US eng J - Článek v odborném periodiku
    Knight, D. - Harvey, P. J. - Iliadi, K. G. - Klose, M. K. - Iliadi, N. - Doleželová, Eva - Charlton, M. P. - Žurovec, Michal - Boulianne, G. L.
    Equilibrative nucleoside transporter 2 regulates olfactory memory and synaptic function in Drosophila.
    Journal of Neuroscience. Roč. 30, č. 14 (2010), s. 5047-5057 ISSN 0270-6474
    Grant CEP: GA MŠk(CZ) LC06077
    Grant ostatní:Canadian Institutes of Health Research(CA) MOP 14143; Canadian Institutes of Health Research(CA) MOP 67130
    Výzkumný záměr: CEZ:AV0Z50070508
    Klíčová slova: Drosophila
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 7.271, rok: 2010

    Nucleoside transporters are evolutionarily conserved proteins that are essential for normal cellular function. In the present study, we examined the role of equilibrative nucleoside transporter 2 (ent2) in Drosophila. Null mutants of ent2 are lethal during late larval/early pupal stages, indicating that ent2 is essential for normal development. Hypomorphic mutant alleles of ent2, however, are viable and exhibit reduced associative learning. We additionally used RNA interference to knock down ent2 expression in specific regions of the CNS and show that ent2 is required in the alpha/beta lobes of the mushroom bodies and the antennal lobes. To determine whether the observed behavioral defects are attributable to defects in synaptic transmission, we examined transmitter release at the larval neuromuscular junction (NMJ). Excitatory junction potentials were significantly elevated in ent2 mutants, whereas paired-pulse plasticity was reduced.
    Trvalý link: http://hdl.handle.net/11104/0194359