Počet záznamů: 1

In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies

  1. 1.
    0355300 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Novotný, Ivan - Blažíková, Michaela - Staněk, David - Heřman, P. - Malínský, Jan
    In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies.
    Molecular Biology of the Cell. Roč. 22, č. 4 (2011), s. 513-523 ISSN 1059-1524
    Grant CEP: GA ČR GA204/07/0133; GA AV ČR KAN200520801
    Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z50390512; CEZ:AV0Z50390703
    Klíčová slova: small nuclear ribonucleoprotein * body components * dynamics
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.942, rok: 2011

    The U4/U6.U5 tri-snRNP is an essential pre-mRNA splicing factor, which is assembled in a step-wise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs) but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching (FRAP). A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. The rates of di-snRNP and tri-snRNP assemblies are similar and ca 230 tri-snRNPs are assembled in one CB per minute. Tri-snRNP assembly is about ten-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems.
    Trvalý link: http://hdl.handle.net/11104/0194107
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