Počet záznamů: 1

The structure of invadopodia in a complex 3D environment

  1. 1.
    0355192 - UMG-J 2011 RIV DE eng J - Článek v odborném periodiku
    Tolde, O. - Rosel, D. - Veselý, Pavel - Folk, P. - Brabek, J.
    The structure of invadopodia in a complex 3D environment.
    European Journal of Cell Biology. Roč. 89, č. 9 (2010), s. 674-680 ISSN 0171-9335
    Grant CEP: GA MŠk(CZ) LC06061
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: invadopodia * invasiveness * SEM
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.630, rok: 2010

    Invadopodia and podosomes have been intensively studied because of their involvement in the degradation of extracellular matrix. As both structures have been studied mostly on thin matrices, their commonly reported shapes and characteristics may differ from those in vivo. To assess the morphology of invadopodia in a complex 3D environment, we observed invadopodial formation in cells grown on a dense matrix based on cell-free dermis. We have found that invadopodia differ in morphology when cells grown on the dermis-based matrix and thin substrates are compared. The cells grown on the dermis-based matrix display invadopodia which are formed by a thick protruding base rich in F-actin, phospho-paxillin, phospho-cortactin and phosphotyrosine signal, from which numerous thin filaments protrude into the matrix. The protruding filaments are composed of an F-actin core and are free of phospho-paxillin and phospho-cortactin but capped by phosphotyrosine signal.
    Trvalý link: http://hdl.handle.net/11104/0194019