Počet záznamů: 1

Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism

  1. 1.
    0355107 - FGU-C 2011 RIV US eng J - Článek v odborném periodiku
    Gabrielová, E. - Jabůrek, Martin - Gažák, Radek - Vostálová, J. - Ježek, Jan - Křen, Vladimír - Modrianský, M.
    Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism.
    Journal of Bioenergetics and Biomembranes. Roč. 42, č. 6 (2010), s. 499-509 ISSN 0145-479X
    Grant CEP: GA ČR(CZ) GA303/08/0658
    Výzkumný záměr: CEZ:AV0Z50110509; CEZ:AV0Z50200510
    Klíčová slova: Reactive oxygen species * Cardiomyocytes * Dehydrosilybin
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 3.637, rok: 2010

    Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging. Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. When investigating the cytoprotective effects of these two compounds in the primary cell cultures of neonatal rat cardiomyocytes, we noted the ability of dehydrosilybin to de-energize the cells by monitoring JC-1 fluorescence. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols
    Trvalý link: http://hdl.handle.net/11104/0193950