Počet záznamů: 1

Mitochondrial phospholipase A2 activated by reactive oxygen species in heart mitochondria induces mild uncoupling

  1. 1.
    0355105 - FGU-C 2011 RIV CZ eng J - Článek v odborném periodiku
    Ježek, Jan - Jabůrek, Martin - Zelenka, Jaroslav - Ježek, Petr
    Mitochondrial phospholipase A2 activated by reactive oxygen species in heart mitochondria induces mild uncoupling.
    Physiological Research. Roč. 59, č. 5 (2010), s. 737-747 ISSN 0862-8408
    Grant CEP: GA ČR(CZ) GA303/07/0105; GA MŠk ME09018; GA AV ČR(CZ) KJB500110902
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: Heart mitochondrial phospholipase A2 * Fatty Acids * Adenine nucleotide translocase
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 1.646, rok: 2010

    Homeostasis of reactive oxygen species in cardiomyocytes is critical for elucidation of normal heart physiology and pathology. Mitochondrial phospholipases A2 have been previously suggested to be activated by ROS. We have found that function of a specific i-isoform of mitochondrial phospholipase A2is activated by tert-butylhydroperoxide in isolated rat heart mitochondria. Concomitant uncoupling has been caused by free fatty acids, since it was inhibited by bovine serum albumin. Since this uncoupling was sensitive to carboxyatractyloside and purine nucleotide di- and tri- phosphates, we conclude that it originated from the onset of fatty acid cycling mediated by the adenine nucleotide translocase (major contribution) and mitochondrial uncoupling protein(s) (minor contribution), respectively. In conclusion, ROS-induced function of cardiac mt-iPLA2 may stand on a pro-survival side of ischemia-reperfusion injury
    Trvalý link: http://hdl.handle.net/11104/0193948