Počet záznamů: 1

Development of soil microbiology methods: from respirometry to molecular approaches

  1. 1.
    0354462 - MBU-M 2011 RIV DE eng J - Článek v odborném periodiku
    Gabriel, Jiří
    Development of soil microbiology methods: from respirometry to molecular approaches.
    Journal of Industrial Microbiology & Biotechnology. Roč. 37, č. 12 (2010), s. 1289-1297 ISSN 1367-5435
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Soil microbiology * Continuous cultivation * Microbial growth
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.416, rok: 2010

    This review deals with techniques and methods used in the study of the function and development of microorganisms occurring in soil with emphasis on the contributions of Czech Academician Ivan Malek and his coworkers or fellows (JiA (TM) i Macura, Frantiek Kunc) to the development of basic techniques used in soil microbiology. Early studies, including batch cultivation and respirometric techniques, as well as later developments of percolation and continuous-flow methods of cultivation of soil microorganisms are discussed. Recent developments in the application of analytical chemistry (HPLC or GC) and of molecular biological techniques to ecological questions that have revolutionized concepts in soil microbiology and microbial ecology are also briefly mentioned, including denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), phospholipid fatty acid analysis (PLFA) and others
    Trvalý link: http://hdl.handle.net/11104/0193458