Počet záznamů: 1

The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons

  1. 1.
    0354390 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Chiu, W.-L. - Wagner, Susan - Herrmannová, Anna - Burela, L. - Zhang, F. - Saini, A. K. - Valášek, Leoš - Hinnebusch, A. G.
    The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons.
    Molecular and Cellular Biology. Roč. 30, č. 18 (2010), s. 4415-4434 ISSN 0270-7306
    Grant CEP: GA ČR GAP305/10/0335
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: 40S RIBOSOMAL-SUBUNIT * SACCHAROMYCES-CEREVISIAE * IN-VIVO
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 6.188, rok: 2010

    The C-terminal domain (CTD) of the a/Tif32 subunit of budding yeast eukaryotic translation initiation factor 3(eIF3) interacts with eIF3 subunits j/Hcr1 and b/Prt1 and can bind helices 16 to 18 of 18S rRNA, suggesting proximity to the mRNA entry channel of the 40S subunit. We have identified substitutions in the conserved Lys-Glu-Arg-Arg (KERR) motif and in residues of the nearby box6 element of the a/Tif32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes indicating defects in scanning and start codon recognition. The normally dispensable CTD of j/Hcr1 is required for its binding to a/Tif32 and to mitigate the growth defects of these a/Tif32 mutants, indicating physical and functional interactions between these two domains
    Trvalý link: http://hdl.handle.net/11104/0193401