Počet záznamů: 1

Protein surface shielding agents in protein crystallization

  1. 1.
    0353319 - UMCH-V 2011 RIV DK eng J - Článek v odborném periodiku
    Hašek, Jindřich
    Protein surface shielding agents in protein crystallization.
    Journal of Synchrotron Radiation. Roč. 18, Part 1 (2011), s. 50-52 ISSN 0909-0495.
    [International Symposium on Diffraction Structural Biology /3./. Paris - Orsay, 25.05.2010-28.05.2010]
    Grant CEP: GA AV ČR IAA500500701; GA ČR GA305/07/1073
    Výzkumný záměr: CEZ:AV0Z40500505
    Klíčová slova: protein adhesion * protein surface shielding agents * intermolecular interactions
    Kód oboru RIV: CD - Makromolekulární chemie
    Impakt faktor: 2.726, rok: 2011

    The molecules adhering temporarily on the surface of protein molecules change the propensity of protein molecules to deposit on the crystal surface in a definite position and orientation. The concepts of competitive adhesion modes and protein surface shielding agents acting on the surface of molecules in a non-equilibrium process of protein crystallization provide a useful platform for the control of crystallization. The desirable goal, i.e. a transient preference of a single dominating adhesion mode between protein molecules during crystallization, leads to uniform deposition of proteins in a crystal. This condition is the most important factor for diffraction quality and thus also for the accuracy of protein structure determination. The presented hypothesis is a generalization of the experimentally well proven behaviour of hydrophilic polymers on the surface of protein molecules of other compounds.
    Trvalý link: http://hdl.handle.net/11104/0192598