Počet záznamů: 1

Distribution and development of the postnatal murine V delta 1 T-cell receptor repertoire

  1. 1.
    0352860 - MBU-M 2011 RIV GB eng J - Článek v odborném periodiku
    Holtmeier, W. - Gille, J. - Zeuzem, S. - Šinkora, Marek
    Distribution and development of the postnatal murine V delta 1 T-cell receptor repertoire.
    Immunology. Roč. 131, č. 2 (2010), s. 192-201 ISSN 0019-2805
    Grant CEP: GA ČR GA524/07/0087
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: gene rearrangement * repertoire development * rodent
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 3.302, rok: 2010

    Murine gamma/delta T cells express canonical V gamma 5V delta 1 chains in the epidermis and V gamma 6V delta 1 chains at reproductive sites. Both subsets carry an identical V delta 1-D delta 2-J delta 2 chain which completely lacks junctional diversity. These cells are thought to monitor tissue integrity via recognition of stress-induced self antigens. In this study, we showed by reverse transcription-polymerase chain reaction (RT-PCR), complementarity determining region 3 (CDR3) spectratyping and sequencing of the junctional regions of V delta 1 chains from C57BL/6 mice (aged 1 day to 14 months) that the canonical V delta 1-D delta 2-J delta 2 chain is also consistently present at other sites such as the thymus, gut, lung, liver, spleen and peripheral blood
    Trvalý link: http://hdl.handle.net/11104/0192264