Počet záznamů: 1

Newly designed, simple relief phase contrast for microscopy of microorganisms

  1. 1.
    0352834 - MBU-M 2011 RIV CZ eng J - Článek v odborném periodiku
    Žižka, Zdeněk
    Newly designed, simple relief phase contrast for microscopy of microorganisms.
    Folia Microbiologica. Roč. 55, č. 6 (2010), s. 662-665 ISSN 0015-5632
    Grant CEP: GA MŠk LC06066
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: optical microscopy * Zeiss NG 10/1 * microphotography
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 0.977, rok: 2010

    A new method providing a relief phase contrast for investigation of microorganisms by optical microscopy used a neutral filter Zeiss NG 10/1 that could be controllably slid at a certain azimuthal angle below the aperture condenser diaphragm of the microscope phase contrast. Two ways of application are described depending on the type of the microscope: (1) in a special holder, and (2) fixed on a rubber ring. The device enabled us to obtain excellent results in the area of both optical microscopy and microphotography. With the microorganisms vizualized, a better resolution, higher contrast and a significant 3D effect were obtained; outer morphology and organelles (chloroplasts, nuclei, granules, oil reserve vacuoles, etc.) could also be investigated
    Trvalý link: http://hdl.handle.net/11104/0192245