Počet záznamů: 1
Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts usány chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry
0352779 - MBU-M 2011 RIV NL eng J - Článek v odborném periodiku
Myjavcová, R. - Marhol, Petr - Křen, Vladimír - Šimánek, V. - Ulrichová, J. - Palíková, I. - Papoušková, B. - Lemr, Karel - Bednář, P.
Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts usány chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry.
Journal of Chromatography A. Roč. 1217, č. 51 (2010), s. 7932-7941 ISSN 0021-9673
Grant CEP: GA MPO FT-TA3/024
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: anthocyanin * blueberry honeysuckle * chromatography
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 4.194, rok: 2010
Anthocyanins from the fruit Lonicera caerulea L. var. kamtschatica (blueberry honeysuckle, Caprifoliaceae) were studied via (semi)preparative chromatographic fractionation followed by MS and LC/MS analysis. The extraction procedure was optimized with respect to analytical purposes as well as its potential use for the preparation of nutraceuticals. The highest yield of anthocyanins was obtained using acidified methanol as the extraction medium. A comparable total anthocyanin content was obtained using a mixture of methanol and acetone. However, when Lonicera anthocyanins were in contact with acetone, a condensation reaction occurred to a large extent and related 5-methylpyranoanthocyanins were found. The effect of other extraction media, including ethanol as a “green” solvent, is also discussed
Trvalý link: http://hdl.handle.net/11104/0192206