Počet záznamů: 1

Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth

  1. 1.
    0352642 - MBU-M 2011 RIV NL eng J - Článek v odborném periodiku
    Kameník, Zdeněk - Hadacek, F. - Marečková, M. - Ulanová, Dana - Kopecký, J. - Chobot, V. - Plháčková, Kamila - Olšovská, Jana
    Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth.
    Journal of Chromatography A. Roč. 1217, č. 51 (2010), s. 8016-8025 ISSN 0021-9673
    Grant CEP: GA MŠk 2B08064
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: UHPLC * high-throughput fingerprinting * secondary metabolites
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 4.194, rok: 2010

    A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector). Thirteen antibiotic standards and four cultivation broths were used for the method development. The comparison of ten liquid–liquid and solid phase extraction protocols for sample clean-up and pre-concentration revealed that Oasis HLB C18 sorbent gives the best recoveries. The Acquity BEH C18 chromatographic column was chosen for the samples separation with respect to its universality, selectivity, efficiency and robustness. The method is presented by two 3D fingerprints for every sample that was obtained under different, acidic and alkaline, UHPLC conditions
    Trvalý link: http://hdl.handle.net/11104/0192110