Počet záznamů: 1

Determination of doxazosin and verapamil in human serum by fast LC-MS/MS: Application to document non-compliance of patients

  1. 1.
    0352169 - UOCHB-X 2011 RIV NL eng J - Článek v odborném periodiku
    Chytil, L. - Štrauch, B. - Cvačka, Josef - Marešová, V. - Widimský jr., J. - Holaj, R. - Slanař, O.
    Determination of doxazosin and verapamil in human serum by fast LC-MS/MS: Application to document non-compliance of patients.
    Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 878, č. 30 (2010), s. 3167-3173 ISSN 1570-0232
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: doxazosin * verapamil * hypertension
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.971, rok: 2010

    A rapid and sensitive method using liquid chromatography–tandem mass spectrometry for simultaneous determination of doxazosin and verapamil in human serum has been developed. Trimipramine-d3 as an isotopic labelled internal standard was used for quantification. Serum samples were prepared by simple liquid–liquid extraction with mixture of tert butyl methyl ether and ethyl acetate. The analytes and internal standard were separated on C18 column using an isocratic elution. Positive TurboIonSpray mass spectrometry was used with multiple reaction monitoring of the transitions. An extensive pre-study method validation was carried out in accordance with FDA guidelines. This assay was successfully applied to determine the serum concentrations of doxazosin and verapamil in suspect non-compliance patients.
    Trvalý link: http://hdl.handle.net/11104/0191745