Počet záznamů: 1

Development of a fast LC-MS/MS method for quantification of rilmenidine in human serum: elucidation of fragmentation pathways by HRMS

  1. 1.
    0352155 - UOCHB-X 2011 RIV GB eng J - Článek v odborném periodiku
    Chytil, L. - Cvačka, Josef - Marešová, V. - Štrauch, B. - Widimský jr., J. - Štícha, M. - Slanař, O.
    Development of a fast LC-MS/MS method for quantification of rilmenidine in human serum: elucidation of fragmentation pathways by HRMS.
    Journal of Mass Spectrometry. Roč. 45, č. 10 (2010), s. 1179-1185 ISSN 1076-5174
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: serum * quantification * HRMS
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 3.289, rok: 2010

    Rilmenidine is an alpha 2 adrenoreceptor agonist used in the treatment of mild and moderate hypertension. A fast and accurate liquid chromatographic method with tandem mass spectrometric detection has been validated in order to assure quantification of rilmenidine in human serum. The fragmentation pathway of protonated rilmenidine was studied using high-resolution mass spectrometry (HRMS). This study compared selectivity, linearity, accuracy, precision, extraction efficiency, matrix effect and sensitivity using common liquid–liquid extraction (LLE) and solid-phase extraction (SPE) procedures. Several differences between the LLE and SPE have been observed in terms of linearity, accuracy, precision and matrix effect. The developed method involving SPE was found to be accurate, reproducible, robust and suitable for quantitative analysis of rilmenidine in serum samples obtained from patients under antihypertensive treatment.
    Trvalý link: http://hdl.handle.net/11104/0191734