Počet záznamů: 1

Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium Synechocystis PCC 6803 with impaired assembly or stability of the CaMn4 cluster

  1. 1.
    0351624 - MBU-M 2011 RIV NL eng J - Článek v odborném periodiku
    Komenda, Josef - Knoppová, Jana - Krynická, Vendula - Nixon, P. J. - Tichý, Martin
    Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium Synechocystis PCC 6803 with impaired assembly or stability of the CaMn4 cluster.
    Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1797, č. 5 (2010), s. 566-575 ISSN 0005-2728
    Grant CEP: GA AV ČR IAA400200801
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: CtpA protease * D1 degradation and maturation * FtsH protease
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 5.132, rok: 2010

    The FtsH2 protease, encoded by the slr0228 gene, plays a key role in the selective degradation of photodamaged D1 protein during the repair of Photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. To test whether additional proteases might be involved in D1 degradation during high rates of photodamage, we have studied the synthesis and degradation of the D1 protein in DPsbO and DPsbV mutants, in which the CaMn4 cluster catalyzing oxygen evolution is less stable, and in the D1 processing mutants, D1-S345P and DCtpA, which are unable to assemble a functional cluster. All four mutants exhibited a dramatically increased rate of D1 degradation in high light compared to the wild type. Additional inactivation of the ftsH2 gene slowed the rate of D1 degradation dramatically and increased the level of PSII complexes. We conclude that FtsH2 plays a major role in the degradation of both precursor and mature forms of D1 following donor-side photoinhibition
    Trvalý link: http://hdl.handle.net/11104/0191336