Localisation of BODIPY-labelled phosphatidylcholines in lipid bilayers

0351463 - ÚFCH JH 2011 RIV GB eng J - Článek v odborném periodiku
Šachl, Radek - Boldyrev, I. - Johansson, L. B. A.
Localisation of BODIPY-labelled phosphatidylcholines in lipid bilayers.
Physical Chemistry Chemical Physics. Roč. 12, č. 23 (2010), s. 6027-6034. ISSN 1463-9076. E-ISSN 1463-9084
Grant CEP: GA ČR GAP208/10/1090
Výzkumný záměr: CEZ:AV0Z40400503
Klíčová slova: phosphatidylcholines * lipid bilayer * electron transfer
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.454, rok: 2010

A series of sn-2 acyl-labelled phosphatidyl-cholines (PC), bearing 4,4-difluoro-1-3-5-7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-8-yl (Me-4-BODIPY) at the end of the C-n-acyl chains were solubilised in unilamellar vesicles and studied with respect to the order and location of the Me4-BODIPY (denoted: B) group. The obtained results are based on time-resolved electronic energy transfer from donors (2-(9-anthroyloxy)-stearic acid) localised in the lipid-water interface to acceptors BnPC (n = 3, 5, 7, 9, 11, 13, 15), as well as the energy migration among the Me4-BODIPY groups of BnPC:s. The donor-acceptor and the donor-donor experiments strongly suggest that the Me4-BODIPY group in BnPC tends to loop back close to the lipid-water interface. The Me4-BODIPY groups, residing in the two bilayer leaflets, are located at approximately the same depth, and transversally separated by ca. 27 angstrom for all n-values.
Trvalý link: http://hdl.handle.net/11104/0191211