Počet záznamů: 1

Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus

  1. 1.
    0351168 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Sklenář, Jan - Niku-Paavola, M. - L. - Santos, S. - Man, Petr - Kruus, K. - Novotný, Čeněk
    Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus.
    Enzyme and Microbial Technology. Roč. 46, č. 7 (2010), s. 550-556 ISSN 0141-0229
    Grant CEP: GA AV ČR IAAX00200901; GA ČR GP310/08/P258; GA MŠk LC06066
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Solid-support growth * White-rot fungus * Irpex lacteus
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.287, rok: 2010

    This paper desribes optimalization of the growth conditions of white-rot fungus Irpex lacteus, a potential candidate for use in bioremediation in situ, on polyurethane solid support. The main objective was isolation and characterization of manganese-dependent peroxidase (MnP) isoenzyme (pI 4.8) exhibitng the highest activity. Optimization of the enzyme production and isolation procedure covering anion exchange chromatography (Mono Q) was performed. The purified protein was subjected to LC-MSMS characterization which provided information on the amino acid sequence as well as the glycosylation
    Trvalý link: http://hdl.handle.net/11104/0190976