Počet záznamů: 1

The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and Cell Plate Maturation

  1. 1.
    0351017 - UEB-Q 2011 RIV US eng J - Článek v odborném periodiku
    Fendrych, Matyáš - Synek, Lukáš - Pečenková, Tamara - Toupalová, Hana - Cole, R. - Drdová, Edita - Nebesářová, Jana - Šedinová, M. - Hála, Michal - Fowler, J.E. - Žárský, Viktor
    The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and Cell Plate Maturation.
    Plant Cell. Roč. 22, č. 9 (2010), s. 3053-3065 ISSN 1040-4651
    Grant CEP: GA MŠk(CZ) LC06034; GA AV ČR KJB600380806; GA AV ČR KJB600380802
    Grant ostatní: GA MŠk ME10033
    Výzkumný záměr: CEZ:AV0Z50380511; CEZ:AV0Z60220518
    Klíčová slova: ELECTRON TOMOGRAPHIC ANALYSIS * SACCHAROMYCES-CEREVISIAE * PLANT TRANSFORMATION
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 9.396, rok: 2010

    Secretory pathways play a crucial role in cytokinesis in plants from the onset of phragmoplast initiation to the maturation of the cell plate. Tethering of secretory vesicles at the plasma membrane is mediated by the exocyst complex. Using proteomic and cytologic approaches, we show that EXO84b is a subunit of the plant exocyst. A. thaliana mutants for EXO84b are severely dwarfed and have compromised leaf epidermal cell and guard cell division. During cytokinesis, green fluorescent protein– tagged exocyst subunits SEC6, SEC8, SEC15b, EXO70A1, and EXO84b exhibit distinctive localization maxima at cell plate initiation and cell plate maturation, stages with a high demand for vesicle fusion. Finally, we present data indicating a defect in cell plate assembly in the exo70A1 mutant. We conclude that the exocyst complex is involved in secretory processes during cytokinesis in Arabidopsis cells, notably in cell plate initiation, cell plate maturation, and formation of new primary cell wall.
    Trvalý link: http://hdl.handle.net/11104/0190861
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