Počet záznamů: 1

Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI

  1. 1.
    0351011 - MBU-M 2011 RIV JP eng J - Článek v odborném periodiku
    Řezanka, Tomáš - Prell, Aleš - Spížek, Jaroslav - Sigler, Karel
    Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI.
    Journal of Antibiotics. Roč. 63, č. 8 (2010), s. 524-529 ISSN 0021-8820
    Grant CEP: GA MŠk 1M06011
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: liquid chromatography/MS-ESI * pilot-plant cultivation * precursor-directed biosynthesis
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 1.628, rok: 2010

    Precursor-directed biogenetic approach was used to produce a range of nonactin homologues in a 50 l fermentor cultivation of strain Streptomyces griseus 34/249 obtained by UV mutagenesis. The production medium contained sodium propionate, isobutyrate and isovalerate as individual precursors, and 10 g l/1 Diaion HP20 styrene-divinylbenzene resin that maintains suitable precursor concentration by reversibly adsorbing and releasing it. The produced nonactin homologues were separated on two C18 reversed-phase liquid chromatography columns in series and analyzed by MS with ESI source in the positive ion mode. Formation of doubly charged ions was suppressed by an excess of Na ions throughout the process. The production of the homologues increased up to day 5 and then it leveled off. Cultivations with individual precursors yielded a total of 18 nonactin homologues whose spectrum depended on the precursor used
    Trvalý link: http://hdl.handle.net/11104/0190856