Počet záznamů: 1

Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1 are involved in the localized deposition of seed coat pectin

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    0350472 - UEB-Q 2011 RIV GB eng J - Článek v odborném periodiku
    Kulich, I. - Cole, R. - Drdová, Edita - Cvrčková, F. - Soukup, A. - Fowler, J. - Žárský, Viktor
    Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1 are involved in the localized deposition of seed coat pectin.
    New Phytologist. Roč. 188, č. 2 (2010), s. 615-625 ISSN 0028-646X
    Grant CEP: GA MŠk ME 841; GA MŠk(CZ) LC06034
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Arabidopsis * cell wall * exocyst
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 6.516, rok: 2010

    Polarized deposition of cell wall pectins is a key process in Arabidopsis thaliana myxospermous seed coat development. The exocyst complex has recently been shown to be involved in the regulation of cell polarity in plants. Here, we used the Arabidopsis seed coat to study the role of the exocyst in polarized pectin delivery. Using a yeast two-hybrid screen, we identified a new interactor of the exocyst subunit Exo70A1, termed Roh1. T-DNA insertions in SEC8, EXO70A1 caused deviations from normal seed coat development, in particular reduced pectin deposition. A gain-of-function mutation of ROH1 also caused reduced pectin deposition. Interestingly, we observed a systematic difference in seed coat development between primary and secondary inflorescences in wild-type plants: siliques from secondary branches produced seeds with thicker seed coats. The participation of exocyst subunits in mucilage deposition provides evidence for the role of the exocyst in polarized cell wall morphogenesis.
    Trvalý link: http://hdl.handle.net/11104/0190472
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