Počet záznamů: 1

Iminodiacetic acid-modified magnetic poly(2-hydroxyethyl methacrylate)-based microspheres for phosphopeptide enrichment

  1. 1.
    0350470 - UMCH-V 2011 RIV NL eng J - Článek v odborném periodiku
    Novotná, L. - Emmerová, T. - Horák, Daniel - Kučerová, Z. - Tichá, M.
    Iminodiacetic acid-modified magnetic poly(2-hydroxyethyl methacrylate)-based microspheres for phosphopeptide enrichment.
    Journal of Chromatography A. Roč. 1217, č. 51 (2010), s. 8032-8040 ISSN 0021-9673
    Grant CEP: GA AV ČR(CZ) KAN401220801; GA ČR GA203/09/0857; GA ČR GAP503/10/0664
    Výzkumný záměr: CEZ:AV0Z40500505
    Klíčová slova: IMAC phosphopeptide separation * IDA-modified magnetic microspheres * Porcine pepsin A
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 4.194, rok: 2010

    Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres prepared by the dispersion polymerization and modified with iminodiacetic acid (IDA) were employed for the IMAC separation of phosphopeptides. Fe3+ and Ga3+ ions immobilized on IDA-modified magnetic microspheres were used for the enrichment of phosphopeptides from the proteolytic digests of two model proteins differing in their physico-chemical properties and phosphate group content: porcine pepsin A and bovine α-casein. The optimum conditions for phosphopeptide adsorption and desorption in both cases were investigated and compared. The phosphopeptides separated from the proteolytic digests were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The ability of the prepared Fe3+- and Ga3+-IDA-modified magnetic microspheres to capture phosphopeptides from complex mixtures was shown on an example of bovine milk proteolytic digest.
    Trvalý link: http://hdl.handle.net/11104/0190470