Transferases and transporters mediate the detoxification and capacity to tolerate trinitrotoluene in Arabidopsis

0350208 - ÚEB 2011 RIV DE eng J - Článek v odborném periodiku
Landa, Přemysl - Štorchová, Helena - Hodek, J. - Vaňková, Radomíra - Podlipná, Radka - Maršík, Petr - Ovesná, J. - Vaněk, Tomáš
Transferases and transporters mediate the detoxification and capacity to tolerate trinitrotoluene in Arabidopsis.
Functional & Integrative Genomics. Roč. 10, č. 4 (2010), s. 547-559. ISSN 1438-793X. E-ISSN 1438-7948
Grant CEP: GA MŠMT 2B06187; GA MŠMT 2B08058
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Microarrays * Arabidopsis thaliana * TNT
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 3.397, rok: 2010

The effect of recalcitrant soil and water pollutant 2,4,6-trinitrotoluen (TNT) on gene expression in Arabidopsis thaliana rosettes and roots was studied separately using microarrays. Seven-day exposure to TNT resulted in 170 up- and 122 down-regulated genes in the rosettes and 61 up- and 51 down-regulated genes in the roots (expression difference>1.5-fold; p[t test]<0.05). TNT concentration, 5 μg/ml, was selected according to the dose response analysis and study of TNT uptake from liquid media. Nitrate reductase 1, several glycosyltransferases and ABC transporters, sucrose–proton symporter 2, thioredoxindependent peroxidase 2, and gamma-glutamyltransferase are discussed for their potential to enhance detoxification and toleration capability of plants to TNT.
Trvalý link: http://hdl.handle.net/11104/0190269