Počet záznamů: 1

Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment

  1. 1.
    0350141 - BC-A 2011 RIV DE eng J - Článek v odborném periodiku
    Chroňáková, Alica - Krištůfek, Václav - Tichý, Martin - Elhottová, Dana
    Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment.
    Microbiological Research. Roč. 165, č. 7 (2010), s. 594-608 ISSN 0944-5013
    Grant CEP: GA AV ČR IAA600660607; GA ČR GA526/03/1259; GA MŠk LC06066; GA MŠk 2B06154
    Grant ostatní: GAJU(CZ) 54/2004/P-BF
    Výzkumný záměr: CEZ:AV0Z60660521; CEZ:AV0Z50200510
    Klíčová slova: actinomycetes * antibiotics * primary succession
    Kód oboru RIV: EH - Ekologie - společenstva
    Impakt faktor: 1.958, rok: 2010

    The genetic diversity of streptomycetes in colliery spoil heaps (Sokolov), Czech Republic) was investigated by restriction pattern analysis of 165-internal transcribed spacer rDNA and 165 sequences. We sampled freshly excavated Miocene sediment (17-19-million-year-old) and four sites of primary succession (initial, early, middle, and late stages; aged 1-44 years) on the same sediment. Active bacteria were present even in fresh Miocene sediment, and the relative proportion of actinomycetes among total bacterial and their genetic diversity increased significantly with the age of the sampling site. The replacement of pioneer species by late succession species during succession was observed. Plate assays of Streptomyces strains revealed 27% antibiotic-producing strains. Screening for nonribosomal peptide synthases and type I polyketide synthases systems suggested that 90% and 55% streptomycetes, respectively, are putative producers of biologically active compounds.
    Trvalý link: http://hdl.handle.net/11104/0190221