Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells

0350113 - ÚEM 2011 RIV GB eng J - Článek v odborném periodiku
Kunová, M. - Matulka, K. - Eiselleová, L. - Trčková, P. - Hampl, Aleš - Dvořák, Petr
Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells.
Reproductive BioMedicine Online. Roč. 21, - (2010), s. 676-686. ISSN 1472-6483. E-ISSN 1472-6491
Grant ostatní: GA MŠk(CZ) LC06077; EC FP6(XE) LSHG-CT-2006-018739
Program: LC
Výzkumný záměr: CEZ:AV0Z50390512; CEZ:AV0Z50390703
Klíčová slova: animal protein-free culture * high-density culture * human embryonic stem cells
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.285, rok: 2010
http://arl-repository.lib.cas.cz/uloziste_av/UEM-P/cav_un_epca-0350113_01.pdf

For human embryonic stem cells (hESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of hESC. Even under the most stringent feeder-free conditions without conditioned medium, hESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype.
Trvalý link: http://hdl.handle.net/11104/0190197