Počet záznamů: 1

Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana

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    0349937 - UEB-Q 2011 RIV GB eng J - Článek v odborném periodiku
    Wimalasekera, R. - Pejchar, Přemysl - Holk, A. - Martinec, Jan - Scherer, G.F.E.
    Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana.
    Molecular Plant. Roč. 3, č. 3 (2010), s. 610-625 ISSN 1674-2052
    Grant CEP: GA MŠk(CZ) LC06034; GA ČR GA522/07/1614
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Auxin * brassinolide signaling * phosphate deficiency
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 4.296, rok: 2010

    Phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphocholine and diacylglycerol (DAG). PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C (PI-PLC). Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes (NPC1 to NPC6) were identified in the Arabidopsis genome. RT-PCR analysis revealed overlapping expression pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-treated P-NPC3:GUS and P-NPC4:GUS seedlings, strong increase of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-treated (BL) seedlings. P-NPC4:GUS seedlings also responded to cytokinin with increased GUS activity in young leaves. Compared to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density.
    Trvalý link: http://hdl.handle.net/11104/0190058
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