Počet záznamů: 1

Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting

  1. 1.
    0349793 - BC-A 2011 RIV NL eng J - Článek v odborném periodiku
    Ascher, J. - Ceccherini, M.T. - Chroňáková, Alica - Jirout, Jiří - Borgogni, F. - Elhottová, Dana - Šimek, Miloslav - Pietramellara, G.
    Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting.
    World Journal of Microbiology and Biotechnology. Roč. 26, č. 9 (2010), s. 1721-1726 ISSN 0959-3993
    Grant CEP: GA MŠk LC06066; GA AV ČR IAA600660605
    Výzkumný záměr: CEZ:AV0Z60660521
    Klíčová slova: cattle impact * DGGE * DGGE interfering factors
    Kód oboru RIV: EH - Ekologie - společenstva
    Impakt faktor: 1.214, rok: 2010

    We compared two denaturing gradient gel electrophoresis (DGGE) systems-DCode (Biorad, Hercules, CA, USA) and PhorU (Ingeny, Leiden, NL), performing community level 16S and 18S rRNA gene fragment-PCR-DGGE with total DNA extracted from upland pasture soil used for outdoor cattle husbandry. The methodological evaluation of the DGGE apparatus as parameter influencing DGGE fingerprinting, based on cluster analysis of soil bacterial and fungal community fingerprints, was made in terms of the resulting information about microbial community structures and their response to different degrees of cattle impact. Although the comparative DGGE analysis with different DGGE systems provided similar clustering of microbial community structures in correlation with the degree of cattle impact, our results suggest the DGGE system to be a factor influencing DGGE analysis.
    Trvalý link: http://hdl.handle.net/11104/0189942