Počet záznamů: 1

Differences in cold adaptation of .i.Bacillus subtilis./i. under anaerobic and aerobic conditions

  1. 1.
    0349691 - BC-A 2011 RIV US eng J - Článek v odborném periodiku
    Beranová, J. - Mansilla, M.C. - de Mendoza, D. - Elhottová, Dana - Konopásek, I.
    Differences in cold adaptation of Bacillus subtilis under anaerobic and aerobic conditions.
    Journal of Bacteriology. Roč. 192, č. 16 (2010), s. 4164-4171 ISSN 0021-9193
    Grant CEP: GA MŠk LC06066
    Výzkumný záměr: CEZ:AV0Z60660521
    Klíčová slova: cold adaptation * Bacillus subtilis * anaerobiosis
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 3.726, rok: 2010

    Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the temperature downshift and is decreased when membrane fluidity is restored. B. subtilis is also capable of anaerobic growth by nitrate or nitrite respiration. We studied the mechanism of cold adaptation in B. subtilis under anaerobic conditions that were predicted to inhibit Des activity. We found that in anaerobiosis, in contrast to aerobic growth, the induction of des expression after temperature downshift (from 37°C to 25°C) was not downregulated. However, the transfer from anaerobic to aerobic conditions rapidly restored the downregulation. However, the transfer from anaerobic to aerobic conditions rapidly restored the downregulation.
    Trvalý link: http://hdl.handle.net/11104/0189855