Počet záznamů: 1

4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet beta-cells, an experimental model of type-2 diabetes

  1. 1.
    0349433 - FGU-C 2011 RIV NL eng J - Článek v odborném periodiku
    Dlasková, Andrea - Špaček, Tomáš - Šantorová, Jitka - Plecitá-Hlavatá, Lydie - Berková, Z. - Saudek, F. - Lessard, M. - Bewersdorf, J. - Ježek, Petr
    4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet beta-cells, an experimental model of type-2 diabetes.
    Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1797, 6-7 (2010), s. 1327-1341 ISSN 0005-2728
    Grant CEP: GA MZd(CZ) NR9183; GA AV ČR(CZ) IAA500110701; GA MŠk(CZ) ME09029
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: 4Pi * diabetes * mitochondrial network morphology
    Kód oboru RIV: ED - Fyziologie
    Impakt faktor: 5.132, rok: 2010

    Pathology of diabetic beta-cells might be reflected by the altered morphology of mitochondrial network. Its characterization is however hampered by the complexity and density of three-dimensional (3D) mitochondrial tubular network in these cell types. Mitochondrial network morphology in fixed cells can be studied by 4Pi microscopy, a laser scanning microscopy technique which provides an 7-fold improved axial resolution (100 nm) over conventional confocal microscopy. We demonstrate that beta-cells within the Langerhans islets from diabetic Goto Kakizaki rats exhibited a more disintegrated mitochondrial network compared to those from control Wistar rats and model insulinoma INS-1E cells
    Trvalý link: http://hdl.handle.net/11104/0189677