Počet záznamů: 1

Magnetic affinity separation of recombinant fusion proteins

  1. 1.
    0348877 - UEK-B 2011 RIV TR eng J - Článek v odborném periodiku
    Šafařík, Ivo - Šafaříková, Miroslava
    Magnetic affinity separation of recombinant fusion proteins.
    Hacettepe Journal of Biology and Chemistry. Roč. 38, č. 1 (2010), s. 1-7 ISSN 1303-5002
    Grant CEP: GA MŠk(CZ) OC 157; GA MPO(CZ) 2A-1TP1/094
    Výzkumný záměr: CEZ:AV0Z60870520
    Klíčová slova: recombinant fusion proteins * affinity tags * magnetic separation
    Kód oboru RIV: CE - Biochemie

    Isolation and separation of proteins is an important procedure in all areas of biosciences and biotechnology. Currently recombinant fusion proteins are produced routinely; in order to simplify their isolation from complex samples, magnetic separation techniques can be efficiently used. This short review paper shows examples of the most important fusion affinity tags and commercially available magnetic affinity and ion exchange particles enabling efficient capture of recombinant fusion proteins. A list of realized isolation procedures documents the efficiency of magnetic separation techniques.
    Trvalý link: http://hdl.handle.net/11104/0189282