RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis

0348166 - MBÚ 2011 RIV US eng J - Článek v odborném periodiku
Schreiberová, O. - Krulikovská, T. - Sigler, Karel - Čejková, A. - Řezanka, Tomáš
RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis.
Lipids. Roč. 45, č. 8 (2010), s. 743-756. ISSN 0024-4201. E-ISSN 1558-9307
Grant CEP: GA MŠMT 2B08062
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Rhodococcus erythropolis * RP-HPLC/MS-APCI * Branched chain triacylglycerols
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.151, rok: 2010

Reversed phase liquid chromatography–atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) was used to analyze both synthetic triacylglycerols (TAG) having 1–3 branched fatty acids (FA) in the molecule, and natural TAG prepared by precursor directed biosynthesis from valine, leucine and isoleucine and the corresponding branched short-chain acids in cultivations of Rhodococcus erythropolis. The technique made it possible to identify and quantify TAG differing in a single branched-chain FA. Altogether 11 TAG were synthesized, out of which 8 were synthesized stereospecifically
Trvalý link: http://hdl.handle.net/11104/0188764