Počet záznamů: 1

RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis

  1. 1.
    0348166 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Schreiberová, O. - Krulikovská, T. - Sigler, Karel - Čejková, A. - Řezanka, Tomáš
    RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis.
    Lipids. Roč. 45, č. 8 (2010), s. 743-756 ISSN 0024-4201
    Grant CEP: GA MŠk 2B08062
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Rhodococcus erythropolis * RP-HPLC/MS-APCI * Branched chain triacylglycerols
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.151, rok: 2010

    Reversed phase liquid chromatography–atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) was used to analyze both synthetic triacylglycerols (TAG) having 1–3 branched fatty acids (FA) in the molecule, and natural TAG prepared by precursor directed biosynthesis from valine, leucine and isoleucine and the corresponding branched short-chain acids in cultivations of Rhodococcus erythropolis. The technique made it possible to identify and quantify TAG differing in a single branched-chain FA. Altogether 11 TAG were synthesized, out of which 8 were synthesized stereospecifically
    Trvalý link: http://hdl.handle.net/11104/0188764