Počet záznamů: 1

Biochemical and Genetic Insights into Asukamycin Biosynthesis

  1. 1.
    0348161 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Rui, Z. - Petříčková, Kateřina - Škanta, František - Pospíšil, Stanislav - Yang, Y. - Chen, Ch.-Y. - Tsai, S.-F. - Floss, H. G. - Petříček, Miroslav - Yu, T.-W.
    Biochemical and Genetic Insights into Asukamycin Biosynthesis.
    Journal of Biological Chemistry. Roč. 285, č. 32 (2010), s. 24915-24924 ISSN 0021-9258
    Grant CEP: GA MŠk 2B06154
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: NODOSUS SUBSP ASUKAENSIS * FATTY-ACID SYNTHASE * CRYSTAL-STRUCTURE
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 5.328, rok: 2010

    Asukamycin, a member of the manumycin family metabolites, is an antimicrobial and potential antitumor agent isolated from Streptomyces nodosus subsp. asukaensis. The entire asukamycin biosynthetic gene cluster was cloned, assembled, and expressed heterologously in Streptomyces lividans. Bioinformatic analysis and mutagenesis studies elucidated the biosynthetic pathway at the genetic and biochemical level. Four gene sets, asuA–D, govern the formation and assembly of the asukamycin building blocks: a 3-amino-4-hydroxybenzoic acid core component, a cyclohexane ring, two triene polyketide chains, and a 2-amino-3-hydroxycyclopent-2-enone moiety to form the intermediate protoasukamycin
    Trvalý link: http://hdl.handle.net/11104/0188761