Počet záznamů: 1

Synthesis of Multivalent Glycoconjugates Containing the Immunoactive LELTE Peptide: Effect of Glycosylation on Cellular Activation and Natural Killing by Human Peripheral Blood Mononuclear Cells

  1. 1.
    0348154 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Renaudet, O. - Křenek, Karel - Bossu, I. - Dumy, P. - Kádek, A. - Adámek, David - Vaněk, O. - Kavan, Daniel - Gažák, Radek - Šulc, Miroslav - Bezouška, K. - Křen, Vladimír
    Synthesis of Multivalent Glycoconjugates Containing the Immunoactive LELTE Peptide: Effect of Glycosylation on Cellular Activation and Natural Killing by Human Peripheral Blood Mononuclear Cells.
    Journal of the American Chemical Society. Roč. 132, č. 19 (2010), s. 6800-6808 ISSN 0002-7863
    Grant CEP: GA MŠk(CZ) LC06010; GA MŠk 1M0505; GA AV ČR IAA400200503; GA ČR GA303/09/0477; GA ČR GD305/09/H008
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: KILLER-CELLS * TN ANTIGEN * RECEPTOR
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 9.019, rok: 2010

    Pentapeptide diacidic sequence LELTE, derived from the mycobacterial heat shock protein hsp65, has been recently identified as a “danger” signal of the immune system effective via specific binding to the universal leukocyte triggering receptor CD69. This sequence is not active per se, only after its presentation within the multivalent environment of its parent protein, or after artificial dimerization using a standard bifunctional reagents. Here we describe an entirely new way of presenting of this peptide based on its attachment to a cyclopeptide RAFT scaffold (K-K-K-P-G)2 through the ε-amino group of lysine residues, alone or in combination with the carbohydrate epitope αGalNAc. The ability of such RAFT scaffolds to precipitate the target CD69 receptor or to activate CD69-positive cells is enhanced in compounds 2 and 4 possessing combined peptide/carbohydrate expression. Compounds 2 and 4 are highly efficient activators of natural killer lymphocytes
    Trvalý link: http://hdl.handle.net/11104/0188757