Počet záznamů: 1

Large-scale separation of silybin diastereoisomers using lipases

  1. 1.
    0348130 - MBU-M 2011 RIV GB eng J - Článek v odborném periodiku
    Gažák, Radek - Marhol, Petr - Purchartová, Kateřina - Monti, D. - Biedermann, David - Riva, S. - Cvak, L. - Křen, Vladimír
    Large-scale separation of silybin diastereoisomers using lipases.
    Process Biochemistry. Roč. 45, č. 10 (2010), s. 1657-1663 ISSN 1359-5113
    Grant CEP: GA ČR(CZ) GA303/08/0658; GA MŠk(CZ) LC06010; GA ČR GAP207/10/0288
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Silybin A * silymarin * silibinin
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.648, rok: 2010

    The flavonolignan silybin (1), isolated from the seeds of milk thistle (Silybum marianum), occurs in nature as an equimolar mixture of two diastereoisomers, silybin A and silybin B, that exhibit different biological activities. The preparative production of optically pure silybin A and B in a diastereoisomeric purity greater than 95 percent was accomplished using immobilized Candida antarctica lipase B (Novozym 435) in a combination of two reactions: regioselective acetylation of a natural silybin mixture (1) and subsequent stereoselective alcoholysis of the resulting 23-O-acetylsilybin (2). Several grams of the optically pure substances can be produced within one week using this new, robust and scalable process, which is selective, mild and high-yielding
    Trvalý link: http://hdl.handle.net/11104/0188740