Počet záznamů: 1

2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing

  1. 1.
    0348081 - UIACH-O 2011 RIV NL eng J - Článek v odborném periodiku
    Petry-Podgorska, Inga - Žídková, Jitka - Flodrová, Dana - Bobálová, Janette
    2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing.
    Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 878, č. 30 (2010), s. 3143-3148 ISSN 1570-0232
    Grant CEP: GA MŠk 1M0570
    Výzkumný záměr: CEZ:AV0Z40310501
    Klíčová slova: 2D-HPLC * MALDI-TOF/TOF mass spectrometry * barley
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 2.971, rok: 2010

    A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called “shot-gun” approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting.
    Trvalý link: http://hdl.handle.net/11104/0188698