2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing

0348081 - ÚIACH 2011 RIV NL eng J - Článek v odborném periodiku
Petry-Podgórska, Inga - Žídková, Jitka - Flodrová, Dana - Bobálová, Janette
2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 878, č. 30 (2010), s. 3143-3148. ISSN 1570-0232. E-ISSN 1873-376X
Grant CEP: GA MŠMT 1M0570
Výzkumný záměr: CEZ:AV0Z40310501
Klíčová slova: 2D-HPLC * MALDI-TOF/TOF mass spectrometry * barley
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.971, rok: 2010

A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called “shot-gun” approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting.
Trvalý link: http://hdl.handle.net/11104/0188698