Počet záznamů: 1

Cytokinin receptor antagonists derived from 6-benzylaminopurine

  1. 1.
    0348001 - UEB-Q 2011 RIV GB eng J - Článek v odborném periodiku
    Nisler, Jaroslav - Zatloukal, Marek - Popa, Igor - Doležal, Karel - Strnad, Miroslav - Spíchal, Lukáš
    Cytokinin receptor antagonists derived from 6-benzylaminopurine.
    Phytochemistry. Roč. 71, č. 7 (2010), s. 823-830 ISSN 0031-9422
    Grant CEP: GA ČR GD522/08/H003; GA ČR(CZ) GP522/07/P197; GA MŠk(CZ) LC06034
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Cytokinin * Anticytokinin * Cytokinin receptor
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 3.150, rok: 2010

    Recently we reported 6-(2-hydroxy-3-methylbenzylamino)purine (PI-55) as the first molecule to antagonize cytokinin activity at the receptor level. Here we report the synthesis and in vitro biological testing of eleven BAP derivatives substituted in the benzyl ring and in the C2, N7 and N9 positions of the purine moiety. The ability of the compounds to interact with Arabidopsis cytokinin receptors AHK3 and CRE1/AHK4 was tested in bacterial receptor and in live-cell binding assays, and in an Arabidopsis ARR5:GUS (Arabidopsis response regulator 5) reporter gene assay. Cytokinin activity of the compounds was determined in classical cytokinin biotests (tobacco callus, wheat leaf senescence and Amaranthus bioassays). 6(2,5-Dihydroxybenzylamino)purine (LGR-991) was identified as a cytokinin receptor antagonist. At the molecular level LGR-991 blocks the cytokinin receptor CRE1/AHK4 with the same potency as PI-55.
    Trvalý link: http://hdl.handle.net/11104/0188632
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