Počet záznamů: 1

Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

  1. 1.
    0347998 - UEB-Q 2011 RIV US eng J - Článek v odborném periodiku
    Luo, M. C. - Ma, Y. Q. - You, F. M. - Anderson, O. D. - Kopecký, David - Šimková, Hana - Šafář, Jan - Doležel, Jaroslav - Gill, B. - McGuire, P. E. - Dvorak, J.
    Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species.
    BMC Genomics. Roč. 11, č. 122 (2010), s. 1-8 ISSN 1471-2164
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: BREAD WHEAT * TETRAPLOID WHEAT * GENETIC-MAP
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.206, rok: 2010

    The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing.
    Trvalý link: http://hdl.handle.net/11104/0188629
    Název souboruStaženoVelikostKomentářVerzePřístup
    2010_Luo_BMC GENOMICS_122.pdf4299.1 KBJinápovolen