Počet záznamů: 1

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

  1. 1.
    0347925 - BC-A 2011 RIV GB eng J - Článek v odborném periodiku
    Takasu, Yoko - Kobayashi, I. - Beumer, K. - Uchino, K. - Sezutsu, H. - Sajwan, S. - Carroll, D. - Tamura, T. - Žurovec, Michal
    Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.
    Insect Biochemistry and Molecular Biology. Roč. 40, č. 10 (2010), s. 759-765 ISSN 0965-1748
    Grant CEP: GA AV ČR IAA500070601; GA ČR GAP305/10/2406; GA MŠk(CZ) LC06077
    Výzkumný záměr: CEZ:AV0Z50070508
    Klíčová slova: gene targeting * nonhomologous and joining * Lepidoptera
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.018, rok: 2010

    The absence of a general gene-targeting system for systematic reverse genetic studies has been a significant limitation in silkworm research. The authors performed direct zinc finger nuclease mRNA injection to /B. mori/ embryos and induced mutations in an epidermal color gene /BmBLOS2. /The results suggest that the /B. mori/ double-strand break repair system relies on microhomologies rather than on a ligase IV-dependent mechanism typical for /Drosophila/. The observed frequency of germline mutations was sufficient for future use in gene targeting relying solely on molecular methods of screening.
    Trvalý link: http://hdl.handle.net/11104/0188585