Počet záznamů: 1

Long-term pioglitazone treatment augments insulin sensitivity and PKC-epsilon and PKC-theta activation in skeletal muscles in sucrose fed rats

  1. 1.
    0347885 - FGU-C 2011 RIV CZ eng J - Článek v odborném periodiku
    Marková, I. - Zídek, Václav - Musilová, Alena - Šimáková, Miroslava - Mlejnek, Petr - Kazdová, L. - Pravenec, Michal
    Long-term pioglitazone treatment augments insulin sensitivity and PKC-epsilon and PKC-theta activation in skeletal muscles in sucrose fed rats.
    Physiological Research. Roč. 59, č. 4 (2010), s. 509-516 ISSN 0862-8408
    Grant CEP: GA MŠk(CZ) 1M0520; GA MŠk(CZ) ME08006; GA AV ČR(CZ) IAA500110604; GA MZd(CZ) NR9387; GA MZd(CZ) NR9359; GA MZd(CZ) NS9759
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: pioglitazone * PKC * insulin resistance
    Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
    Impakt faktor: 1.646, rok: 2010

    It has been suggested that thiazolidinediones (TZDs) ameliorate insulin resistance in muscle tissue by suppressing muscle lipid storage and the activity of novel protein kinase C (nPKC) isoforms. To test this hypothesis, we analyzed long-term metabolic effects of pioglitazone and the activation of nPKC-epsilon and -theta isoforms in an animal model of the metabolic syndrome, the spontaneously hypertensive rat (a congenic SHR strain with wild type Cd36 gene) fed a diet with 60 % sucrose from the age of 4 to 8 months. Pioglitazone-treated rats exhibited significantly increased membrane/total (cytosolic plus membrane) ratio of both PKC-epsilon and PKC-theta isoforms compared to untreated controls. These results suggest that amelioration of insulin resistance after long-term pioglitazone treatment is associated with increased activation of PKC-epsilon and -theta isoforms in spite of increased lipid concentration in skeletal muscles
    Trvalý link: http://hdl.handle.net/11104/0188558