Počet záznamů: 1

An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part II: oxidative damage to DNA

  1. 1.
    0347658 - UEM-P 2011 RIV IE eng J - Článek v odborném periodiku
    Rössner ml., Pavel - Topinka, Jan - Hovorka, J. - Milcová, Alena - Schmuczerová, Jana - Kroužek, J. - Šrám, Radim
    An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part II: oxidative damage to DNA.
    Toxicology Letters. Roč. 198, č. 3 (2010), s. 312-316 ISSN 0378-4274
    Grant CEP: GA MŠk 2B08005
    Grant ostatní: GA MŽP(CZ) SP/1A3/149/08
    Výzkumný záměr: CEZ:AV0Z50390512
    Klíčová slova: air pollution * particulate matter * atmospheric aerosol
    Kód oboru RIV: DN - Vliv životního prostředí na zdraví
    Impakt faktor: 3.581, rok: 2010

    The study investigated the ability of organic extracts of size segregated aerosol particles-PM (EOM; three size fractions ranging from 0.17 to 10µm and collected in 4 localities differing in the extent of pollution) to induce oxidative damage to DNA in an acellular system of calf thymus DNA with/without metabolic activation. It was found that levels of 8-oxodG tended to increase with decreasing sizes of PM; metabolic activation increased the oxidative capacity of PM. A significant positive association between concentrations of c-PAHs bound to PM and levels of 8-oxodG after metabolic activation of EOM samples was observed. The correlation was weaker and non-significant for samples without metabolic activation. It may be concluded that organic extracts of PM were able to induce oxidative damage to DNA in vitro; this ability was increased after S9 metabolic activation of EOM and with decreasing sizes of PM.
    Trvalý link: http://hdl.handle.net/11104/0188390