Počet záznamů: 1

Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells

  1. 1.
    0347619 - UEB-Q 2011 RIV GB eng J - Článek v odborném periodiku
    Laňková, Martina - Smith, R. S. - Pešek, Bedřich - Kubeš, Martin - Zažímalová, Eva - Petrášek, Jan - Hoyerová, Klára
    Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells.
    Journal of Experimental Botany. Roč. 61, č. 13 (2010), s. 3589-3598 ISSN 0022-0957
    Grant CEP: GA AV ČR KJB600380702; GA MŠk(CZ) LC06034
    Grant ostatní: _(CZ) CZ.2.16/3.1.00/21159
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Auxin efflux carrier * auxin influx carrier * auxin transport
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 4.818, rok: 2010

    Synthetic auxins that differ in the mechanisms of their transport across the plasma membrane together with polar auxin transport inhibitors have been used in many studies on particular auxin carriers and their role in plant development. In this report, the mechanism of action of the auxin influx inhibitors (1-naphthoxyacetic acid (1-NOA), 2-naphthoxyacetic acid (2-NOA), and 3-chloro-4-hydroxyphenylacetic acid (CHPAA)) is examined by direct measurements of auxin accumulation, cellular phenotypic analysis, as well as by localization studies of Arabidopsis thaliana auxin carriers heterologously expressed in Nicotiana tabacum BY-2 cell suspensions. The mode of action of 1-NOA, 2-NOA, and CHPAA has been shown to be linked with the dynamics of the plasma membrane. The most potent inhibitor, 1-NOA, blocked the activities of both auxin influx and efflux carriers, whereas 2-NOA and CHPAA at the same concentration preferentially inhibited auxin influx.
    Trvalý link: http://hdl.handle.net/11104/0188363
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