Počet záznamů: 1

YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei

  1. 1.
    0347288 - BC-A 2011 RIV NL eng J - Článek v odborném periodiku
    Týč, Jiří - Long, Shaojun - Jirků, Milan - Lukeš, Julius
    YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei.
    Molecular and Biochemical Parasitology. Roč. 173, č. 1 (2010), s. 43-47 ISSN 0166-6851
    Grant CEP: GA ČR GA204/09/1667
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Trypanosoma * Plastid * Mitochondrion * Targeting * YCF45 * Horizontal gene transfer
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.875, rok: 2010

    YCF45 belongs to a family of proteins of unknown function usually located in the chloroplast of plants. Its highly conserved homologues were found in the genomes of several Trypanosoma and Leishmania species. HA(3)-tagging of the YCF45 protein with the start codon as annotated in the Gene(DB) revealed its cytosolic localization in the cultured procyclic stage of Trypanosoma brucei. However, when a more upstream located start codon was used in another HA(3)-tagged construct, the resulting protein was targeted to the mitochondrion. We propose that YCF45 was acquired by an ancestral trypanosomatid by horizontal gene transfer and in the absence of a plastid was re-targeted to the mitochondrion.
    Trvalý link: http://hdl.handle.net/11104/0188095