Počet záznamů: 1

Benzimidazole resistance allele haplotype diversity in United Kingdom isolates of Teladorsagia circumcincta supports a hypothesis of multiple origins of resistance by recurrent mutation

  1. 1.
    0347281 - BC-A 2011 RIV AU eng J - Článek v odborném periodiku
    Skuce, P. - Stenhouse, L. - Jackson, F. - Hypša, Václav - Gilleard, J.
    Benzimidazole resistance allele haplotype diversity in United Kingdom isolates of Teladorsagia circumcincta supports a hypothesis of multiple origins of resistance by recurrent mutation.
    International Journal for Parasitology. Roč. 40, č. 11 (2010), s. 1247-1255 ISSN 0020-7519
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: BZ resistance * b-Tubulin * Haplotype diversity * Haplotype diversity
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.822, rok: 2010

    Polymorphisms in the isotype 1 beta-tubulin gene are important genetic determinants of benzimidazole (BZ) resistance in a number of parasitic nematode species including Teladorsagia circumcincta, a major gastrointestinal nematode of sheep. This study investigates the genetic diversity at this locus in a BZ-resistant Isolate of T circumcincta (MTc15) derived from a sheep farm in UK that was open to animal, and therefore parasite, migration. Pyrosequencing was used to determine the frequency of single nucleotide polymorphisms (SNPs) known to be associated with BZ resistance. This was followed by a combination of single strand conformation polymorphism (SSCP) analysis and nucleotide sequencing to sample allelic diversity in a 276 bp fragment immediately surrounding the isotype I beta-tubulin F200Y mutation The genetic diversity at this locus was extremely high, with seven different haplotypes found to contain the resistant F200Y polymorphism in this single resistant isolate.
    Trvalý link: http://hdl.handle.net/11104/0188090