Počet záznamů: 1

Protein structure-sensitive electrocatalysis at dithiothreitol-modified electrodes

  1. 1.
    0347221 - BFU-R 2011 RIV US eng J - Článek v odborném periodiku
    Ostatná, Veronika - Černocká, Hana - Paleček, Emil
    Protein structure-sensitive electrocatalysis at dithiothreitol-modified electrodes.
    Journal of the American Chemical Society. Roč. 132, č. 27 (2010), s. 9408-9413 ISSN 0002-7863
    Grant CEP: GA AV ČR(CZ) KJB100040901; GA ČR(CZ) GP202/07/P497; GA MŠk(CZ) LC06035
    Grant ostatní: GA AV ČR(CZ) KAN400310651
    Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Klíčová slova: constant current chronopotentiometry * mercury and solid amalgam electrodes * structure-sensitive protein analysis
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 9.019, rok: 2010

    Dithiothreitol (DTT)-mercury and DTT-solid amalgam electrodes are proposed for protein microanalysis by means of constant current chronopotentiometric stripping (CPS). Self-assembled monolayers (SAMs) of DTT at the electrode surface protected surface-attached proteins from the electric field-driven denaturation. Using CPS peak H, native and denatured forms of bovine serum albumin and of other proteins were easily distinguished.
    Trvalý link: http://hdl.handle.net/11104/0188047