Počet záznamů: 1

Heterochromatin marks HP1gamma, HP1alpha and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours

  1. 1.
    0347140 - UMG-J 2012 RIV GB eng J - Článek v odborném periodiku
    Bartkova, J. - Moudrý, Pavel - Hodný, Zdeněk - Lukas, J. - Rajpert-De Meyts, E. - Bartek, Jiří
    Heterochromatin marks HP1gamma, HP1alpha and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours.
    International Journal of Andrology. Roč. 34, 4 Pt 2 (2011), e103-e113 ISSN 0105-6263
    Grant CEP: GA ČR GA301/08/0353
    Grant ostatní: Lundbeck Foundation(DK) R13-A1287; EU FP7(XE) TRIREME 223575
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: heterochromatinization * DNA damage response * germinal tumours
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.591, rok: 2011

    Heterochromatinization has been implicated in fundamental biological and pathological processes including differentiation, senescence, ageing and tumorigenesis; however, little is known about its regulation and roles in human cells and tissues in vivo. Here, we show distinct cell-type- and cancer-stage-associated patterns of key heterochromatin marks: histone H3 trimethylated at lysine 9 (H3K9me3) and heterochromatic adaptor proteins HP1α and HP1γ, compared with the γH2AX marker of endogenously activated DNA damage response (DDR) and proliferation markers in normal human foetal and adult testes, pre-invasive carcinoma in situ (CIS) lesions and a series of overt germ cell tumours, including seminomas, embryonal carcinomas and teratomas. Among striking findings were high levels of HP1γ in foetal gonocytes, CIS and seminomas; enhanced multimarker heterochromatinization without DDR activation in CIS; and enhanced HP1α in teratoma structures with epithelial and neuronal differentiation.
    Trvalý link: http://hdl.handle.net/11104/0187984