Počet záznamů: 1

Regulation of the PML tumor suppressor in drug-induced senescence of human normal and cancer cells by JAK/STAT-mediated signaling

  1. 1.
    0347137 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Hubáčková, Soňa - Nováková, Zora - Krejčíková, Kateřina - Košař, Martin - Dobrovolná, Jana - Dušková, Pavlína - Hanzlíková, Hana - Vančurová, Markéta - Barath, P. - Bartek, J. - Hodný, Zdeněk
    Regulation of the PML tumor suppressor in drug-induced senescence of human normal and cancer cells by JAK/STAT-mediated signaling.
    Cell Cycle. Roč. 9, č. 15 (2010), s. 3085-3099 ISSN 1538-4101
    Grant CEP: GA AV ČR IAA500390501; GA ČR GA204/08/1418; GA ČR GA301/08/0353
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: PML tumor suppressor * cellular senescence * JAK-STAT signaling pathway
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.999, rok: 2010

    PML tumor suppressor is upregulated in several forms of cellular senescence, however the mechanism of its induction is elusive. Here we show that genotoxic drugs that induce senescence, such as bromodeoxyuridine, thymidine, distamycin A, aphidicolin, etoposide and camptothecin evoke expansion of PML nuclear compartment and its association with persistent DNA lesions in several human normal and cancer cells. This phenomenon was accompanied by elevation of PML transcripts. Chemical inhibition of all JAK kinases and RNAi-mediated knock-down of JAK1 suppressed PML expression, implicating JAK/STAT-mediated signaling in regulation of the PML gene. Our data show that upregulation of the PML tumor suppressor in cellular senescence triggered by diverse drugs including clinically used anti-cancer chemotherapeutics relies on stimulation of PML transcription by JAK/STAT-mediated signaling, possibly evoked by the autocrine/paracrine activities of senescence-associated cytokines.
    Trvalý link: http://hdl.handle.net/11104/0187982