Počet záznamů: 1

RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription

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    0347094 - UMG-J 2011 RIV GB eng J - Článek v odborném periodiku
    Kanagaraj, R. - Huehn, D. - Mackellar, A. - Menigatti, M. - Zheng, L. - Urban, Václav - Shevelev, Igor - Greenleaf, A.L. - Janščák, Pavel
    RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription.
    Nucleic Acids Research. Roč. 38, č. 22 (2010), s. 8131-8140 ISSN 0305-1048
    Grant CEP: GA ČR GA204/09/0565
    Grant ostatní: SNSF(CH) 3100A0-116008; NIH(US) GM040505
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: RECQ5 DNA helicase * transcription * genome stability
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 7.836, rok: 2010

    It is known that transcription can induce DNA recombination, thus compromising genomic stability. RECQ5 DNA helicase promotes genomic stability by regulating homologous recombination. Recent studies have shown that RECQ5 forms a stable complex with RNA polymerase II (RNAPII) in human cells, but the cellular role of this association is not understood. Here, we provide evidence that RECQ5 specifically binds to the Ser2,5-phosphorylated C-terminal repeat domain (CTD) of the largest subunit of RNAPII, RPB1, by means of a Set2–Rpb1-interacting (SRI) motif located at the C-terminus of RECQ5. We also show that RECQ5 associates with RNAPII-transcribed genes in a manner dependent on the SRI motif. Notably, RECQ5 density on transcribed genes correlates with the density of Ser2-CTD phosphorylation, which is associated with the productive elongation phase of transcription.
    Trvalý link: http://hdl.handle.net/11104/0187948
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