Počet záznamů: 1

The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells

  1. 1.
    0347089 - UMG-J 2011 RIV CH eng J - Článek v odborném periodiku
    Čermák, Vladimír - Kosla, Jan - Plachý, Jiří - Trejbalová, Kateřina - Hejnar, Jiří - Dvořák, Michal
    The transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells.
    Cellular and Molecular Life Sciences. Roč. 67, č. 20 (2010), s. 3557-3568 ISSN 1420-682X
    Grant CEP: GA AV ČR KAN200520801; GA MŠk(CZ) LC06061; GA ČR(CZ) GA204/07/1030
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: smooth muscle cells * endothelial kinase * Rho kinase
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 7.047, rok: 2010

    To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines-the highly metastatic parental PR9692 and a non-metastasizing but fully tumorigenic clonal derivative PR9692-E9. Oligonucleotide microarray analysis of both cell lines revealed that the gene encoding the transcription factor EGR1 was downregulated in the non-metastatic PR9692-E9 cells. Further investigation demonstrated that the introduction of exogenous EGR1 into PR9692-E9 cells restored their metastatic potential to a level indistinguishable from parental PR9692 cells. Microarray analysis of EGR1 reconstituted cells revealed the activation of genes that are crucial for actin cytoskeleton contractility (MYL9), filopodia formation (MYO10), the production of specific extracellular matrix components (HAS2, COL6A1-3) and other essential pro-metastatic abilities.
    Trvalý link: http://hdl.handle.net/11104/0187946
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