Počet záznamů: 1

Identification of Carbonic Anhydrase I Immunodominant Epitopes Recognized by Specific Autoantibodies Which Indicate an Improved Prognosis in Patients with Malignancy after Autologous Stem Cell Transplantation

  1. 1.
    0347082 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Skultety, L. - Jankovičová, B. - Svobodová, Z. - Mader, Pavel - Řezáčová, Pavlína - Dubrovčáková, M. - Lakota, J. - Bílková, Z.
    Identification of Carbonic Anhydrase I Immunodominant Epitopes Recognized by Specific Autoantibodies Which Indicate an Improved Prognosis in Patients with Malignancy after Autologous Stem Cell Transplantation.
    Journal of Proteome Research. Roč. 9, č. 10 (2010), s. 5171-5179 ISSN 1535-3893
    Grant CEP: GA MŠk 1M0505; GA ČR GA203/09/0820
    Grant ostatní: BITCET(CZ) SPVV 337/2003; EEA(NO) SK 0095; TRANSMED(XE) 2624012008
    Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z40550506
    Klíčová slova: epitope mapping * carbonic anhydrase I * spontaneous remission
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 5.460, rok: 2010

    Spontaneous tumor regression was observed in patients who developed aplastic anaemia type syndrome after high-dose therapy with autologous stem cell transplantation and whose sera contained high titer of anti-carbonic anhydrase (anti-CA) autoantibodies. Many indications suggest that presence of these autoantibodies provides significant survival benefit for the patients. Western blot analysis confirmed immunoreactivity of the patients’ sera with several CA isoforms. Highly abundant and widely distributed CA I was selected for epitope mapping study. The applied analytical approach consisted of specific fragmentation of CA I followed by immunospecific isolation of peptides reacting with polyclonal anti-CA I autoantibodies. Standard epitope mapping schema was improved by incorporating magnetic carriers and biomagnetic separation techniques. Mass spectrometry was used for detection and identification of epitopes and acquired results were verified by bioinformatic tools.
    Trvalý link: http://hdl.handle.net/11104/0187939